Tissue Micro Array

Tissue Micro Array (TMA)

The Pathology Core can construct custom TMA's for researchers who have large collections of their tissue of interest and would like to be able to perform "high-throughput" analysis.

Why Work With Small Samples?

  • Preserve valuable tissue
  • Increase efficiency cost savings for expensive reagents
    • Less antibody
    • "High throughput"
  • Large populations = interesting questions    

Why Do I Need a TMA?

  • Basic research
    • Validate and verify gene expression data
    • Translational research                                                                           
      • Diagnostic markers
      • Prognostic markers
      • Therapeutic markers
      • Response to therapy
      • Query signaling pathways
      • Drug discovery
  • Tissue is a valuable resource

What Do I Need to Make a TMA?

  • Source tissue
    • Animal tissue from experimental models
    • Clinical archival tissue
    • Prospectively acquired clinical tissue
    • Associated data
  • Pathologist
    • Confirm diagnosis
    • Select representative areas for sampling
      • TMA cores are representative
        • 20 studies to date on a variety of tumors have shown strong correspondence in IHC results on TMAs when compared to "large" sections (n=3-4 cores)
    • Validate the constructed array
    • Score the array
    • Antibody or in situ probe to query array
      • Phosphorylation state specific antibodies
      • Signaling pathways
    • Data management and analysis tools
    • Willingness to have many collaborators

    TMA data analysis

    • IHC scoring
      • 0 negative
      • 1 indeterminate
      • 2 weakly positive
      • 3 strongly positive
    • Scores for each individual core in TMA are collected for every stain performed
    • Large data sets
    • Expression data can be analyzed by bioinformatic methods developed for cDNA microarrays
    • Quantitative scoring (pixel counts/percent area stained) can also be performed as well

    Arrays Previously Constructed:

    Array DescriptionPrimary InvestigatorInstitution
    Neurodegenerative DiseaseMark FormanUPENN
    NeuroblastomaJohn MarisCHOP
    MeduloblastomaMariarita Santi-ViciniCHOP
    Developmental MouseJeff GoldenCHOP
    Lung TumorSteve AlbeldaUPENN
    AT/RTMariarita Santi-ViciniCHOP
    LymphomaRebecca KingCHOP
    Small Round Blue CellLisa SullivanMSU
    EpendymomaMariarita Santi-ViciniCHOP
    AstrocytomaMariarita Santi-ViciniCHOP
    RhabdomyocarcomaDiana CoraoCHOP/Dupont
    Bone Fracture ArrayTheresa FreemanTJU
    Thyroid Tumor ArrayBruce PawelCHOP
    Normal Tissue ArrayTricia BhattiCHOP
    Neuroepithelial ArrayMariarita Santi-ViciniCHOP
    Breast Cancer ArrayJuan PallazzoTJU

     

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